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3-in-1 Plate

Hydrogel-Based Culture Plate Insert



  • Create spheroids, apply extracellular matrices, test drugs and perform downstream analysis. All without ever having to transfer the spheroids from one platform to another. 

  • Uniform spheroids for experimental consistency.

  • No risk of losing spheroids during media changes.

  • Lower cell requirements reduces the need to passage cells for organoid formation.  

  • One spheroid per microwell. No crosstalk between the models. 

  • Easily image spheroids in situ.

  • Spheroids can be formalin fixed, paraffin embedded, and sliced directly within the hydrogel platform, enabling in situ IHC analysis


The 3-in-1 Plate is a hydrogel insert that fits within each well of a six-well plate. The 3-in-1 Plate combines spheroid formation, ECM integration, drug testing and downstream analysis all in one easy-to-use platform. The insert is perfectly suited to various assays and is compatible with standard laboratory imaging equipment, making it suitable to a wide range of experiments. 

  • Material: Transparent Biocompatible Hydrogel.

  • 3-in-1 Plate Specifications:

    • Number of spheroids per insert: 24

    • Number of spheroids per six-well plate: 144

    • Volume of each microwell: 1 mm^3

    • Spheroid size range: 200 - 500 μm


  • Invasion studies

  • Co-culture studies

  • Matrix stiffness studies (compatible with all ECM's)

  • Drug screening

  • Immunohistochemistry

  • Spatial Biology


  • Inserts must be stored at 4 C in a refrigerator while not in use. 

  • Pre-sterilized. 

  • Handle with care when removing the 3-in-1 Plate inserts from wells. 

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