Spheroids can be formed from various cell types using methods like hanging drop plates, low-attachment surfaces, micropatterned substrates, and rotating bioreactors. Among these, multicellular tumor spheroids (MCTS) derived from cancer cells are the gold standard in cancer research. MCTS replicate the complex architecture of solid tumors, with a proliferative outer layer, a quiescent middle zone shaped by nutrient and oxygen gradients, and a necrotic core. This organization captures key tumor features such as cellular heterogeneity, nutrient diffusion, waste clearance, and drug penetration, making MCTS highly predictive and physiologically relevant models.Unlike traditional two-dimensional cultures, 3D spheroids better mimic in vivo cellular interactions and microenvironments. Their simplicity, versatility, and cost-effectiveness support high-throughput screening, making them essential for studying tumor biology, metastasis, and drug response. Apricell’s 3D tumor spheroid platforms harness these strengths to provide scalable, robust, and clinically relevant models for personalized cancer research and therapy development.